DAILAB-CAFÉ 系列讲座 通知
讲座题目：Cell-based assay system to monitor the circadian clock and its application for screening physiologically active substances from natural products
演讲人： Tatsunosuke TOMITA
演讲人单位：National Institute of Advanced Industrial Science & Technology (AIST), Japan
讲课时间： 3月9日 15：00 -16：00
演讲内容：Circadian rhythm is a ~24 h cycle of physiological process, observed widely in many creatures from cyanobacteria to mammalian cells. In the molecular level, this phenomenon is mainly controlled by 4 components of clock genes, namely clock, bmal1, pers and crys. They comprise the transcriptional and translational feedback loop and control the expression of many genes rhythmically. We and other researchers developed cell-based assay systems for monitoring circadian clock. These systems are based on the luciferin-luciferase real-time reporter gene assay.
In this talk, I will present:
1) Review of the molecular mechanisms of the circadian clock.
2) Effective methods for monitoring the molecular clock in the cellular level.
3) Application of this monitoring system to screen compounds effective to circadian rhythm from natural products.Through this session, I would discuss merits of applying the bioluminescence system to monitor the biological clock physiology.